3. DNA Extraction - Logistics

Time
40 minutes

Grouping
individual

Materials
Each student needs:

  • 1 fresh strawberry (frozen strawberries also work fine although they are not nearly as much fun to eat)
  • 1 ziplock bag
  • 1 15 ml centrifuge tube or 5 oz paper bathroom cup
  • 1 clear glass or plastic test tube
  • 1 paper towel
  • 10 ml extraction buffer (see recipe below)
  • 1 bamboo skewer or glass stirring rod (DNA tends to stick more fiercely to bamboo than wood – however, bamboo is MUCH cheaper)

Extraction buffer recipe:

  • 450 ml distilled water
  • 10 g table salt
  • 50 ml Dawn dishwashing detergent

Optional for making necklaces:

  • 1 1.5 ml Eppendorf tube (also known as microcentrifuge tubes, available from Science Kit and Boreal Labs at $13.25 for 500 tubes)
  • 50 cm of string or yarn

For the whole class to share:

  • clean food bowl
  • clean toothpicks for eating strawberries
  • 90% ice cold rubbing alcohol or ethanol
  • eye droppers for dispensing solutions

Setting
Classroom